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| Modern Genetic Analysis Chapter 13. Transposable Genetic Elements Transposition through an RNA Intermediate Figure 13-20. Demonstration of transposition through an RNA intermediate. A Ty element is altered by adding a promoter that can be activated by the addition of galactose. Activation of the promoter will increase transcription through the Ty element. Then an intron from another gene is inserted into the Ty element. Because the final product of transposition contains no intron, the intron must have been spliced out of an RNA transcript (see Chapter 3). This splicing must have occurred as shown here, where the primary transcript contains the intron but the final processed mRNA does not. This RNA is then copied by reverse transcriptase and integrated into the chromosomal DNA. (Modified from H. Lodish, D. Baltimore, A. Berk, S. L. Zipursky, P. Matsudaira, and J. Darnell, Molecular Cell Biology, 3d ed. p. 332. Copyright © 1995 by Scientific American Books).

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