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Modern Genetic Analysis Chapter 7. Gene Mutations The Molecular Basis of Mutation
Table 7-2. Forward Mutation Frequencies Obtained with Various Mutagens in Neurospora

Mutagenic treatment | Exposure time (minutes) | Survival (%) | Number of ad-3 mutants per 106 survivors

No treatment (spontaneous rate) |  | 100 | ~0.4
Amino purine (15 mg/ml) | During growth | 100 | 3
Ethyl methane sulfonate (1%) | 90 | 56 | 25
Nitrous acid (0.05 m) | 160 | 23 | 128
X rays (2000 r/min) | 18 | 16 | 259
Methyl methane sulfonate (20 mm) | 300 | 26 | 350
UV rays (600 erg/mm2 per min) | 6 | 18 | 375
Nitrosoguanidine (25 m m) | 240 | 65 | 1500
ICR-170 acridine mustard (5 m g/ml) | 480 | 28 | 2287

NOTE: The assay measures the frequency of ad-3 mutants. It so happens that such mutants are red, so they can be detected against a background of white ad-3+ colonies.

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