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| Modern Genetic Analysis Chapter 12. Genomics Structural Genomics Figure 12-15. (a) Pulsed field gel electrophoresis of uncut chromosomal DNA from different strains of yeast. Sixteen bands are resolved. Because there are 22 chromosomes in yeast, some have obviously comigrated in the gel. (All lanes were loaded identically.) (b) Using pulsed field gel electrophoresis to separate Neurospora chromosomes and to locate a cloned gene onto one chromosomal segment: (left) the seven chromosomes of wild type and a translocation in which a 100-kb section of chromosome II has been inserted into chromosome III; (right) autoradiogram with the use of a probe consisting of a cloned gene of previously unknown location. The results show that the gene is located on the region of chromosome II that is moved in the translocation. (Part a from Bio-Rad Laboratories; part b from Myron Smith.)

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