Book Viewer

Short Contents | Full Contents | Other books @ NCBI

| Modern Genetic Analysis Chapter 14. Regulation of Gene Transcription Transcription: Gene Regulation in Eukaryotes an Overview Figure 14-20. Consequences of point mutations in the promoter for the b -globin gene. Point mutations through the promoter region were analyzed for their effects on transcription rates. The height of each line represents the transcription level, relative to a wild-type promoter, that results from promoters with base changes at that point. A level of 1.0 means that the rates are equal to the wild-type rate; reductions in transcription rates yield levels less than 1.0. Almost every nucleotide throughout the promoter was tested, except for the points shown with black dots. The diagram below the bar graph shows the position of the TATA box and two upstream elements of the promoter. Only the base substitutions that lie within the three promoter elements change the level of transcription. (From T. Maniatis, S. Goodbourn, and J. A. Fischer, Science 236, 1987, 1237.)

© 1999 by W. H. Freeman and Company. All rights reserved.