Book Viewer

Short Contents | Full Contents | Other books @ NCBI

| Modern Genetic Analysis Chapter 16. The Genetic Basis of Development Sex Determination in Mammals: Coordinated Control by the Endocrine System Genetics In Process 16-2: The mutational analysis of mammalian sex determination A transgenic mouse that proves that Sry can cause the sex-reversal syndrome. (a) Amplification of genomic DNA by the polymerase chain reaction (PCR) shows that mouse 33.13 lacks a DNA marker for the presence of a Y chromosome (Zfy-1) but that it does contain the Sry transgene. M = marker bands. (b) The external genitalia of sex-reversed XX transgenic mouse 33.13 are indistinguishable from those of a normal XY male sib (33.17), demonstrating that the Sry gene is sufficient to cause the sex-reversal phenotype. (From P. Koopman, J. Gubbay, N. Vivian, P. Goodfellow, and R. Lovell-Badge, Nature 351, 1991, 117.)

© 1999 by W. H. Freeman and Company. All rights reserved.