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| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Making Recombinant DNA Figure 10-2. Plasmids such as those carrying genes for resistance to the antibiotic tetracycline (top left) can be separated from the bacterial chromosomal DNA. Because differential binding of ethidium bromide by the two DNA species makes the circular plasmid DNA denser than the chromosomal DNA, the plasmids form a distinct band on centrifugation in a cesium chloride gradient and can be separated (bottom left). They can then be introduced into bacterial cells by transformation (right). (Modified from S. N. Cohen, "The Manipulation of Genes." Copyright © 1975 by Scientific American, Inc. All rights reserved.)

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