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| Modern Genetic Analysis Chapter 11. Applications of Recombinant DNA Technology Recombinant DNA Technology in Eukaryotes Figure 11-10. Simplified representations of four different kinds of plasmids used in yeast. Each is shown acting as a vector for some genetic region of interest, which has been inserted into the vector. The function of such segments can be studied by transforming a yeast strain of suitable genotype. Selectable markers are needed for the routine detection of the plasmid in bacteria or yeast. Origins of replication are sites needed for the bacterial or yeast replication enzymes to initiate the replication process. (DNA derived from the 2- m m natural yeast plasmid has its own origins of replication.)

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