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| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Using Cloned DNA Figure 10-19. An example of a Southern analysis. The DNA from the mitochondria of 11 strains of Neurospora from different geographical locations was electrophoresed, and the gel was stained with ethidium bromide. The bright bands are linear plasmids, and the positions of some of these plasmids are marked. The lane marked "L" is a calibration ladder composed of DNA fragments of known size. The sizes of the rungs visible in this figure are (from the bottom up) 1.5 kb, 2 kb, and then one additional kilobase for each rung, up to 12 kb. The plasmid in strain 11 was cloned and used as a probe in a Southern hybridization. The autoradiogram shows that several other plasmids have sequences homologous to the strain 11 plasmid. Indeed, strain 7 has a family of three plasmids, all homologous to the strain 11 plasmid. (From Simon Yang.)

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