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Modern Genetic Analysis Chapter 10. Recombinant DNA Technology
Solved Problem

3. | From Table 10-1, determine whether the EcoRI enzyme or the SmaI enzyme would be more useful for cloning. Explain your answer. See answer

Solution

Both restriction enzymes recognize a six-base-pair sequence, so both would be expected to have approximately the same number of recognition sites per genome. The major difference between the two is that EcoRI leaves staggered ends, whereas SmaI leaves blunt ends. Staggered ends are much easier to manipulate during cloning because of the base-pairing capacity inherent in them.

(Solution from Diane K. Lavett.)

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