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| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Cloning a Specific Gene Figure 10-8. Cloning by cosmids. The cosmid is cut at a BglII site next to the cos site. Donor genomic DNA is cut by using Sau3A, which gives sticky ends compatible with BglII. A tandem array of donor and vector DNA results from mixing. Phage is packaged in vitro by cutting at the cos site. The cosmid with insert recircularizes after it is in the bacterial cell. (From J. D. Watson, M. Gilman, J. Witkowski, and M. Zoller, Recombinant DNA, 2d ed. Copyright © 1992 by Scientific American Books.)

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