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Modern Genetic Analysis Chapter 11. Applications of Recombinant DNA Technology
Solved Problem

1.

DNA studies are performed on a large family that shows a certain autosomal dominant disease of late onset (approximately 40 years of age). A DNA sample from each family member is digested with the restriction enzyme TaqI and subjected to gel electrophoresis. A Southern blot is then performed, with the use of a radioactive probe consisting of a part of human DNA cloned in a bacterial plasmid. The autoradiogram, aligned with the family pedigree, is as follows. Affected members are shown in black.

a. Analyze fully the relation between the DNA variation, the probe DNA, and the gene for the disease. Draw the relevant chromosome regions.
b. How do you explain the last son?
c. Of what use would these results be in counseling people from this family who subsequently married?
See answer

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