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| Modern Genetic Analysis Chapter 11. Applications of Recombinant DNA Technology Recombinant DNA Technology in Eukaryotes Figure 11-15. (a) To produce transgenic plants, an intermediate vector of manageable size is used to clone the segment of interest. In the method shown here, the intermediate vector is then recombined with an attenuated ("disarmed") Ti plasmid to generate a cointegrate structure bearing the insert of interest and a selectable plant kanamycin-resistance marker between the T-DNA borders, which is all the T-DNA that is necessary to promote insertion. (b) The generation of a transgenic plant through the growth of a cell transformed by T-DNA.

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