Book Viewer

Short Contents | Full Contents | Other books @ NCBI

| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Cloning a Specific Gene Figure 10-6. Two plasmids designed as vectors for DNA cloning, showing general structure and restriction sites. Insertion into pBR322 is detected by inactivation of one drug-resistance gene (tetR), indicated by the tetS (sensitive) phenotype. Insertion into pUC18 is detected by inactivation of the b -galactosidase function of Z, resulting in an inability to convert the artificial substrate X-Gal into a blue dye.

© 1999 by W. H. Freeman and Company. All rights reserved.