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| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Using Cloned DNA Figure 10-22. The dideoxy sequencing method. (a) A labeled primer (designed from the flanking vector sequence) is used to initiate DNA synthesis. The addition of four different dideoxy nucleotides (ddATP is shown here) randomly arrests synthesis. (b) The resulting fragments are separated elec-trophoretically and subjected to autoradiography. (From J. D. Watson, M. Gilman, J. Witkowski, and M. Zoller, Recombinant DNA, 2d ed. Copyright © 1992 by Scientific American Books.)

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