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Modern Genetic Analysis Answers to Selected Problems
Chapter 10*

2. | GTTAAC occurs, on average, every 46 bases, which is 4.096 kb. GGCC occurs, on average, every 44 bases, which is 0.256 kb. See question

3. | See question

5. | Reading from the top, the sequence is See question

7. | a. b. See question

9. | Use the vector of the cloned Neurospora gene as a probe. Prepare a library of Podospora genomic DNA or cDNA by using the plasmid shown. Insert Podospora DNA into a unique site: the only one shown in the plasmid is the HaeII site in the kanR gene. Therefore the first screen will be for tet-resistant colonies, which will be the ones with vector. These colonies will be tested to see which are kanamycin sensitive because they will carry the inserts. The tetR kanS colonies will be probed to see which contains the specific tubulin gene. See question

10.

a. There is one BglII site, and the plasmid is 14 kb.
b. There are two EcoRV sites.
c. The arrangement of the sites must be as follows.
d. The BglII site must be within the tetR site.
e. There was an insert at the BglII site in the clone.
f. There was an EcoRV site within the insert.
See question

15. | a and b. See question

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