Book Viewer

Short Contents | Full Contents | Other books @ NCBI

| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Cloning a Specific Gene Figure 10-7. Cloning in phage l . A nonessential central region of the phage chromosome is discarded and the ends ligated to random 15-kb fragments of donor DNA. A linear multimer (concatenate) forms, which is then stuffed into phage heads one monomer at a time by using an in vitro packaging system. (From J. D. Watson, M. Gilman, J. Witkowski, and M. Zoller, Recombinant DNA, 2d ed. Copyright © 1992 by Scientific American Books.)

© 1999 by W. H. Freeman and Company. All rights reserved.