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| Modern Genetic Analysis Chapter 12. Genomics Structural Genomics Figure 12-28. Using an ordered array of YACs to locate the map position of a newly cloned gene in Caenorhabditis elegans. The YAC clones are placed in order on a polytene filter. DNA from this filter is blotted and probed with the cloned gene, giving the autoradiogram with two positive spots corresponding to two adjacent YACs numbered 332 and 333. Because of the hybridization pattern obtained, the location of the cloned gene can be narrowed down to a small region on chromosome III. (Autoradiogram from Alan Coulson.)

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