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| Modern Genetic Analysis Chapter 10. Recombinant DNA Technology Cloning a Specific Gene Figure 10-12. Finding the clone of interest by using antibody. An expression library made with phage derivative l gt11 is screened with a protein-specific antibody. After the un-bound antibodies have been washed off the filter, the bound antibodies are visualized through binding of a radioactive secondary antibody. (From J. D. Watson, M. Gilman, J. Witkowski, and M. Zoller, Recombinant DNA, 2d ed. Copyright © 1992 by Scientific American Books.)

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